Label-Free Super-Resolution Microscopy by Vasily Astratov

Author:Vasily Astratov
Language: eng
Format: epub, pdf
ISBN: 9783030217228
Publisher: Springer International Publishing

In order to test the super-resolving capabilities of the proposed method, a resolution target containing three bars, as illustrated in Fig. 9.5a, was used. The size of the each bar was set to match the dimensions of the modified PSF . As mentioned earlier, the inspected sample was illuminated with a pump beam at wavelength of λ = 750 nm and, as a result, the shape of the PSF was modified. This is presented in Fig. 9.5b. The sample was convolved with this PSF . The resulting low-resolution image, in which the three resolution bars are not seen, is presented in Fig. 9.5c.

Fig. 9.5Simulation images of the a resolution target, b the modified shape of the PSF , c low-resolution image and d the deconvolved image. e The sample analysis carried out with a Gaussian PSF shape.

Reproduced from [28]

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